FIG 4.
Identification of VraSRSS-regulated genes via transcriptional analysis. (A) Comparison of gene regulation by RNA-Seq analysis or qRT-PCR. qRT-PCR was used to validate the expression level changes of 10 selected genes, including 4 upregulated genes and 6 downregulated genes, identified by RNA-Seq analysis. Each sample was run in triplicate, and the reference gene parC was detected as a control. Relative fold changes in expression were calculated using the 2−ΔΔCT method. (B) The VraSRSS-regulated genes identified by transcriptional analysis were involved in CPS biosynthesis. The expression levels of CPS biosynthesis-related genes were significantly lower in the ΔvraSRSS strain.