FIG 6.
VraSRSS is involved in SS2 adhesion to bEnd.3 cells and resisting phagocytosis by RAW 264.7 cells. (A) Effect of the vraSRSS mutation on the ability of SS2 to adhere to bEnd.3 cells. The adhesion rate of the ZY05719 strain was significantly lower than that of the ΔvraSRSS and Δcps strains. The vraSRSS-complemented strain showed adhesion restored to the level of the WT strain. (B) Effect of the vraSRSS deletion on the ability of SS2 to resist phagocytosis by RAW 264.7 cells. The antiphagocytosis rate of the ZY05719 strain was significantly higher than that of the ΔvraSRSS and Δcps strains. The vraSRSS-complemented strain showed an ability restored to the level of the WT strain. The data are shown as the means and standard deviations of the results from three independent experiments performed in triplicate. Two-tailed unpaired Student's t tests were used for statistical analysis (***, P < 0.001).