FIG 5.

iNOS or reactive oxygen species do not compensate for arginase-1 deficiency in myeloid cells. S. aureus orthopedic implant infections were established in Arg-1^fl/fl;Tie-2^null (WT) or Arg-1^fl/fl;Tie-2^Cre mice (A to C) or in C57BL/6 mice (D to F), whereupon animals received daily treatments of apocynin (6 mg/kg) (A to C), l-NIL (40 mg/kg) (A to C), or DFMO (50 mg/kg) (D to F) beginning 5 h prior to infection. Bacterial burdens in surrounding soft tissues (A and D), the femur (B and E), and the knee joint (C and F) were quantified at the indicated time points postinfection. *, P < 0.05; ***, P < 0.001 (unpaired 2-tailed Student's t test).