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. 2018 Jun 21;86(7):e00183-18. doi: 10.1128/IAI.00183-18

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Copyright © 2018 Bolick et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license.

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FIG 2 — Intestinal inflammation induced by ETEC strain H10407 infection in antibiotic-pretreated mice fed a nourished diet (HC), a protein-deficient diet (dPD), or a zinc-deficient diet (dZD). Protein extracts were obtained from fecal samples at day 2 postinfection by lysing fecal specimens in RIPA buffer. Supernatants from the lysates were used for protein quantification and specific ELISAs for evaluation of myeloperoxidase (MPO) or lipocalin (LCN-2). (A) LCN-2 fecal levels after infection. *, P < 0.05 (dZD versus dZD plus ETEC and dPD versus dPD plus ETEC). (B) MPO fecal levels after infection. *, P < 0.05 (HC versus HC plus ETEC and dPD versus dPD plus ETEC); **, P < 0.01 (dZD versus dZD plus ETEC) (n = 8/group).