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. 2018 Jun 21;86(7):e00183-18. doi: 10.1128/IAI.00183-18

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Copyright © 2018 Bolick et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license.

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FIG 5 — Zinc supplementation increases ETEC H10407 intestinal colonization in zinc-deficient infected C57BL/6 mice. Zinc-deficient mice received supplemental zinc sulfate in drinking water (150 mg/liter) starting from day 12 post-ETEC infection. Quantitative PCR was performed using DNA samples that had been extracted from stool samples or intestine samples from ETEC-infected mice. (A) Stool ETEC H10407 shedding before zinc treatment. (B) Effect of zinc treatment on stool ETEC H10407 shedding after 13 days of zinc treatment (day 25 postinfection). *, P < 0.05 (dZD versus dZD plus Zn). (C) Effect of zinc treatment on colon and ileum ETEC H10407 colonization after 13 days of zinc treatment (day 25 postinfection). *, P < 0.05 (dZD versus dZD plus Zn in the colon) (n = 4/group). (D) Effect of zinc treatment on cecal ETEC H10407 toxin expression following zinc treatment. *, P = 0.0008 (no Zn versus Zn).