FIG 6.

Outcomes of infection with an ETEC LT mutant of strain H01407 (LTKO) are different from those seen with the wild type in house chow-deficient and zinc-deficient mice. House chow-deficient or zinc-deficient mice were infected with either the ETEC H10407 wild type or ETEC LTKO strains. Daily weight measures were collected from the day of infection until the end of the experiment. (A) Percentages of body weight change of house chow mice after infection. (B) Percentages of body weight change of zinc-deficient mice after infection. *, P < 0.05 (ZD versus ZD plus ETEC WT/LTKO). (C) Shedding of ETEC WT and LTKO strains in HC- and dZD-fed mice. LTKO ETEC showed a trend of lower shedding levels than WT ETEC following 1 week of infection in both diet groups. There were no significant differences between groups. (D) Myeloperoxidase (MPO) fecal levels after 6 days of infection. Protein extracts were obtained from fecal samples at day 2 postinfection by lysing fecal specimens in RIPA buffer. Supernatants from the lysates were used for protein quantification and specific ELISAs for evaluation of MPO. *, P < 0.05 (HC plus ETEC WT versus HC ETEC LTKO) (n = 8/group).