TABLE 2.
Primers used in this study
| Group/primer | Primer sequence 5′ to 3′ | Location (accession no.) |
|---|---|---|
| gspB or hsa | ||
| SRRP.1 | TCGGATCCAGAATTCGAAGCAATGCCAAAGTGTAGGa | 648–668 (AY028381.5) |
| SRRP.2 | CACGAACGAAAATCGTCCAAATTGAGATGTAGCCGCb | 1244–1224 (AY028381.5) |
| SRRP.3 | ATAAACCCTTGCATAGGAGCTTTGGGATTGGCGTTb | 10299–10318 (AY028381.5) |
| SRRP.4 | CTTGTCGACGAATTCACGATGGACTTAATGGTGGTTa | 10804–10784 (AY028381.5) |
| GspB.5 | CTCATTTACGGAGCAAGAAAAG | 507–528 (AY028381.5) |
| GspB.6 | TATGCAAGGGTTTATTGTTTTC | 1156–1135 (M69221.1) |
| GspB.i | TTGGAATCTGTATACTGGTG | 333807–333826 (JYGL01000001.1) |
| GspB.ii | TACTTGAGTCACAGAATACTGC | 334212–334191 (JYGL01000001.1) |
| GspB.iii | TCAATAAATTTACTTCCGTC | 334622–334603 (JYGL01000001.1) |
| GspB.iv | GAAATGTAAATGTAACTCGG | 334307–334326 (JYGL01000001.1) |
| GspB.v | TAGTATCTATCAGTGGTAATC | 333716–333736 (JYGL01000001.1) |
| GspB.vi | GGGCCCCTTTCCTTATGCTT | 2284–2265 (MF927926.1) |
| Hsa.5 | CGATTTTCGTTCGTGAATAC | 3–22 (M69221.1) |
| Hsa.6 | AATAGCGCAGGAAAACTGTAT | 1018861–1018841 (CP000725.1) |
| SRRP.7 | TGTTTGACTAGAAGCTTCTGTCG | 1847–1825 (AY028381.5) |
| SRRP.8 | TACTAATCTGAAGATCTTTAACTAC | 1013044–1013020 (CP000725.1) |
| bgaA | ||
| B.1 | TCGGATCCAGAATTCCATTAACAGGTACAGAGCTTAGa | 1543379–1543358 (CP000725.1) |
| B.2 | CACGAACGAAAATCGTCCGCATAAACAGGATTTCCTGb | 1542873–1542894 (CP000725.1) |
| B.3 | ATAAACCCTTGCATAGAAGCAGTGCAGCTTCAAGCAGb | 1536115–1536094 (CP000725.1) |
| B.4 | CTTGTCGACGAATTCAGTAATATTGACTAGAGGCACAGa | 1535560–1535582 (CP000725.1) |
| B.5 | ATAAAGATCCTAAAGATCCAGG | 1543511–1543490 (CP000725.1) |
| B.6 | TATGCAAGGGTTTATTGTTTTC | 1156–1135 (M69221.1) |
| B.i | CTGCGACAAATGAAGAAG | 410–427 (KX792092.1) |
| B.ii | GTTTGGATGTTTCGCACGAAG | 1914–1894 (KX792092.1) |
| B.iii | ATGGACAGCAATCACATTC | 837–819 (KX792092.1) |
| B.iv | CGTCTCAAACAAATGAAG | 1402–1419 (KX792092.1) |
| B.v | GGAATCGTAAAAGAGTCTAC | 17–36 (KX792092.1) |
| B.vi | GGGCCCCTTTCCTTATGCTT | 2284–2265 (MF927926.1) |
| srtA | ||
| S.1 | TCGGATCCAGAATTCCTGATTAATGTTTTCCTAACTGATa | 1276706–1276683 (CP000725.1) |
| S.2 | CACGAACGAAAATCGTTTCTTCTTGGCTCTTCGTGAb | 1276155–1276175 (CP000725.1) |
| S.3 | ATAAACCCTTGCATATACTTCAATAAATCATACAATCAAb | 1275467–1275444 (CP000725.1) |
| S.4 | CTTGTCGACGAATTCGTTCAAATAAAGTGCAACCAAATGa | 1274955–1274978 (CP000725.1) |
| S.5 | CGATTTTCGTTCGTGAATAC | 3–22 (M69221.1) |
| S.6 | CCATCATTTCGCAGGCTTCTT | 1274747–1274767 (CP000725.1) |
| nanA | ||
| N.5 | GGTGAGGTCAAGATGATTACG | 505512–505532 (FR720602.1) |
| N.6 | AACGGCCGCCAGTGTGCTG | 319–336 (EU233623) |
| TZ86_00548 | ||
| TZ86_00548F | TGGCTGCTCAAGATTTCWATGc | 560435–560415 (JYGL01000001.1) |
| TZ86_00548F2 | CTYCGTAATGAAGCAAAAGd | 558420–558402 (JYGL01000001.1) |
| TZ86_00548R | AACAGATGTAAAGGTTGGAG | 558018–558038 (JYGL01000001.1) |
| aad9 | ||
| S.F | CGATTTTCGTTCGTGAATAC | 3–22 (M69221.1) |
| S.R | TATGCAAGGGTTTATTGTTTTC | 1156–1135 (M69221.1) |
| Janus | ||
| J.F | CCGTTTGATTTTTAATGGATAATG | 773–796 (AF411920.1) |
| J.R | GGGCCCCTTTCCTTATGCTT | 2105–2086 (AF411920.1) |
| pDrive | ||
| T7 promoter | GTAATACGACTCACTATAG | 239–258 (DQ996013) |
| M13F | GTAAAACGACGGCCAGT | 431–447 (DQ996013) |
a
Underlining indicates nucleotides introduced to allow in-fusion cloning into the pDrive vector.
b
Underlining indicates nucleotides introduced to allow in-fusion cloning with aad9 (spectinomycin cassette).
c
W = A or T.
d
Y = C or T.