FIG 1.

Measurement of neutrophils during a Plasmodium berghei ANKA infection in ECM-susceptible C57BL/6 and -resistant BALB/c mice. Mice were infected with 10⁶ Plasmodium berghei ANKA (Pb-A) parasites. (A to D) Parasitemia was enumerated in the two strains of mice (A), and neutrophils were quantitated by measuring the expression of CD11b and Ly6G on live lymphocyte singlets in spleen tissue (B) and plotted as proportion (C) and absolute number (D) over the course of a strain ANKA infection. (E to G) Neutrophils were also quantitated in perfused brain tissue (E), and the percentage (F) and absolute number (G) of brain-sequestered leukocytes that were CD11b⁺ Ly6G⁺ neutrophils was also determined in perfused brain tissue of susceptible C57BL/6 mice on days 0, 3, and 6 postinfection and resistant BALB/c mice on day 6 postinfection. n = 5 (spleen) and n = 4 (brain) for each time point for each strain of mouse, and results presented are representative of three independent experiments.