FIG 2.

Plasmodium berghei ANKA induces expression of TCRβ on neutrophils in C57BL/6 and BALB/c mice. Mice were infected with 10⁶ Plasmodium berghei ANKA (Pb-A) parasites. The proportion of neutrophils that were TCRβ⁺ CD3ε⁻ (A) was then determined and graphed as proportion (B) and absolute number (C) during a strain ANKA infection in C57BL/6 and BALB/c mice. (D) TCRβ and CD3ε expression was also measured on brain-sequestered neutrophils of susceptible C57BL/6 mice on days 0, 3, and 6 postinfection and resistant BALB/c mice on day 6 postinfection. Findings are presented as proportion (E) and absolute number (F). A fluorescence-minus-one control for TCRβ was used for gating purposes. n = 5 (spleen) and n = 4 (brain) for each time point for each strain of mouse, and results presented are representative of three independent experiments.