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. 2001 Oct 9;98(22):12820–12825. doi: 10.1073/pnas.221198698

Figure 1.

Figure 1

Identification of peptide synthetase sequences linked to C. purpurea dmaW. (A ) PCR with cosmids containing C. purpurea American Type Culture Collection (ATCC) 20102 dmaW as template and primers designed to polypeptide sequences GKPKG and YKTGDL [based on conserved regions in peptide synthetase genes (25)] results in the expected ≈700-bp product from dmaW-containing cosmids 26–7A (cos26), 27–1A (cos27), and 29–5A (cos29). [Cosmid 39–2F (cos39) contains a dmaW pseudogene; it does not appear to contain a peptide synthetase gene.] (B) The PCR product generated from cosmid 27–1A in A hybridizes with EcoRI fragments of the cosmid from which it was generated and also with Cp605, previously cloned from C. purpurea ATCC 34501 but not with any of the other peptide synthetase gene fragments previously cloned and characterized from ergopeptine-producing fungi (25, 35). Fragment sizes (in kb) indicate the relative mobility of fragments of BstEII-digested bacteriophage λ.