Fig. 5. Binding assay for wt AhbD using different substrate analogs. The binding of (a) Fe-COPRO III, (b) COPRO III, (c) Cu-COPRO III and (d) Zn-COPRO III to wt AhbD was followed by UV-visible absorption spectroscopy. The spectra were recorded after 24 h of incubation after mixing the protein with the respective substrate analog. The shift of the Soret bands upon AhbD addition is indicated.
