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. 2016 Apr 5;7(7):4016–4023. doi: 10.1039/c5sc03796d

Table 1. Ru(ii)–dppz complexes/PCP can be used as live-cell DNA probes with many unsurpassed characteristics, compared with the current commonly used organic DNA probes a .

Ru(ii)–dppz a /PCP DAPI Hoechst 33342
Excitation/emission maximum (nm) 445/620 358/461 b 350/461 b
Stokes shift >150 103 111
TEM +
Photo-bleaching Low High (1–2 min) High
UV damage + +
Enantioselectivity +
Cell permeable + –/+ +
Living cell/fixed cell +/+ +/+ +/+
Binding mode Intercalator c MGB/AT preference b MGB/AT preference b
Working concentration 80 μg ml–1 0.5–10 μg ml–1 0.25–20 μg ml–1

aRu(ii)–dppz: [Ru(bpy)2(dppz)]2+ and [Ru(phen)2(dppz)]2+.

bMolecular probes.