Fig. 5. Confocal images of Cou–DEVD–TPETP (10 μM) incubated HeLa cells upon treatment with (A) sodium ascorbate (Na Asc), (B) cisplatin, (C) DOX and (D) STS. Green fluorescence (Cou–DEVD, Ex: 405 nm; Em: 505–525 nm); orange fluorescence (nucleus dyed with SYTO® orange, Ex: 543 nm, Em: 610–640 nm); A1–D1 are the overlay images of the fluorescence of Cou and SYTO® orange; red fluorescence (TPETP residue, A2–D2, Ex: 405 nm, Em: >650 nm). (E) PL intensities of Cou–DEVD and the TPETP residue in HeLa cells treated with Na Asc, cisplatin, DOX and STS at different concentrations.
