Fig. 5. (A) An aqueous solution of DFS containing 5% DMF as co-solvent can conveniently convert commercially available phage displayed library of 109 disulfide heptapeptides—Ph.D. C7C from New England Biolabs—to a library of OFS-macrocyclic peptides. (B) Biotin capture, analogous to that described in Fig. 4C–E, quantified the efficiency of this modification. After 30 minutes to 2 hours reaction with DFS, 35–45% of the library is modified with DFS yielding ∼350–450 million of diverse phage-displayed OFS-macrocycles. Data is averaged from two independent experiments.
