Table 1.
Impaired MTN afferent bifurcation in the absence of Npr2 or CNP.
| CONTROLS AND GLOBAL KNOCKOUTS | |||||||
|---|---|---|---|---|---|---|---|
| CNP+/+ | CNPLacZ/LacZ | Npr2+/+ and Npr2CreERT2/+;Z/AP | Npr2LacZ/LacZ and Npr2CreERT2/cn;Z/AP | ||||
| Control | Knockout | Control | Knockout | ||||
| Bifur | Turn | Bifur | Turn | Bifur | Turn | Bifur | Turn |
| 7 (3) | 0 (3) | 3 (2) | 26 (2) | 8 (2) | 0 (2) | 1 (2) | 14 (2) |
| CONTROL AND CONDITIONAL KNOCKOUTS | |||||||
| Npr2flox/flox | Npr2flox/flox; Wnt1Cre | Npr2flox/flox; Engr1Cre | |||||
| Control | Co.knockout | Co.knockout | |||||
| Bifur | Turn | Bifur | Turn | Bifur | Turn | ||
| 2 (3) | 0 (3) | 0 (3) | 14 (3) | 1 (5) | 27 (5) | ||
Total numbers of individual MTN axons that could be identified by DiI tracing or sparse labeling using the Npr2CreERT2;Z/AP transgene. The first number indicates the number of individual axons identified and the second number in parenthesis the numbers of embryos analyzed. In control animals (CNP+/+, Npr2+/+, Npr2CreERT2/+;Z/AP and Npr2flox/flox) 17 bifurcating and 0 non-bifurcating (“turn”) axons were counted in 8 embryos. In homozygous mutants (CNPLacZ/LacZ, Npr2LacZ/LacZ, Npr2CreERT2/cn;Z/AP, Npr2flox/flox;Wnt1Cre and Npr2flox/flox;Engr1Cre) 5 bifurcating and 81 non-bifurcating axons were counted in 12 embryos.