Fig. 4.

The molecular mechanisms underlying tractional retinal detachment.

(a–d) Real-time PCR analyses of PDGF ligands. Pdgfb mRNA (b) expression at 2 weeks was exclusively and significantly higher in N-PRβ-KO mice compared to that in WT mice. Pdgfa (a), Pdgfc (c), and Pdgfd (d) mRNA expression levels in N-PRβ-KO mice were similar or lower than the levels observed in WT mice. n = 8 at the indicated time points. (E and F) ELISA data for retinas at 1 to 4 weeks. PDGF-AB (e) expression was comparable between the two genotypes. PDGF-BB (f) expression in N-PRβ-KO was significantly higher at 2 weeks and tended to be higher at 4 weeks than that of WT mice. n = 5–7 at the indicated time points. (g and h) Real-time PCR analyses of PDGFRs expression. Pdgfra mRNA (g) expression in N-PRβ-KO was significantly higher at 2 weeks and tended to higher at 4 weeks than that of WT mice. Pdgfrb mRNA (h) expression in N-PRβ-KO was significantly lower at all time points than those in WT mice. n = 8 at the indicated time points. (i and j) Western blotting data of retinas at 2 weeks. PDGFRα protein (i) expression was significantly higher in N-PRβ-KO than that in WT mice. PDGFRβ protein (j) expression was significantly low level in N-PRβ-KO compared to WT mice. n = 5 in both genotypes. All values represent means ± SEM. *, p < 0.05; **, p < 0.01; ***, p < 0.001 vs. WT mice at the same time points. ND indicates not detected.