Fig. 2.

Gene expression of NbTM8 and NbSVP in Nicotiana benthamiana. a Relative expression of NbTM8 in vegetative and reproductive structures of two biological replicates. b–c In situ hybridisation of NbTM8 in developing shoots and flowers. d Developmental series from cotyledons (cot) to 14 leaves (L14) of NbTM8. Gray arrow marks the floral transition, the black arrow marks the floral induction. e Relative expression of miR156 (left) and miR172 (right) during Nicotiana development. f Yeast two-hybrid of NbTM8 and NbSVP. g Co-IP results for NbTM8 and NbSVP. Proteins were pulled down with FLAG coupled agarose beads and visualised by HA (up) and FLAG (down) antibodies through Western Blot. h Relative expression of NbSVP in vegetative and reproductive structures. i–j In situ hybridisation of NbSVP in developing shoots and flowers. k Developmental series of NbSVP. sam: shoot apical meristem; f: flower meristem; LD: long day. Error bars are standard deviations