Table 1.

List of strains and plasmids used in this study

Strains	Characteristics	Origin	Disease	References
LF82	Wild type AIEC O83:H1 AmpR	Human	Crohn’s disease	[4]
LF82ΔnagC	LF82 ΔnagC AmpR KanR			This study
MG1655	Laboratory E. coli K-12	Human	Healthy	[47]
EDL933	Wild type EHEC O157:H7	Human	Diarrhea, HUS	[48]
17.2	Wild type EAEC	Human	Diarrhea	[49]
NC101	Wild type commensal (AIEC-like)	Mouse	Mouse colitis	[50]
χ7213	thi-1 thr-1 leuB6 glnV44 fhuA21 lacY1 recA1 RP4-2-Tc::Mu λpir ΔasdA4 Δzhf-2::Tn10			[51]

Plamids	Characteristics	References
pGEM®-T	Cloning vector	Promega Corp., Madison, WI, USA
pMEG-375	Suicide vector sacRB mobRP4 oriR6K; CmR ApR	Roy Curtiss III, Arizona State University, Tempe, AZ, USA
pΔnagC	pMEG-375 with ΔnagC:: KmR	[16]
pTrc99a	expression vector with IPTG inducible lacI promoter; ApR	[52]
pnagC	pTrc99a with nagC	[16]
pnagC-JFS	pnagC with CmR	This study