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. 2018 Jun 21;17:96. doi: 10.1186/s12943-018-0844-7

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© The Author(s). 2018

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 2 — miR-25 knockdown promotes OvCa cell migration. a The expression of miR-25 in OvCa cells after treatment with 10 ng/ml TGF-β1 for 48 h. b The mRNA levels of EMT-related markers in SKOV3 cells transfected with a miR-25 inhibitor (AMO-25) or a negative control (NC). c Western blotting analysis of epithelial and mesenchymal markers in SKOV3 cells transfected with AMO-25. Wound-healing (d) and migration assays (e) were used to determine the effect of miR-25 inhibition on OvCa cell migration. n = 5 independent experiments. *P < 0.05, **p < 0.01 vs. NC