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Tandem fluorescent protein timer (tfT) approach to measure cytosolic protein turnover by the ubiquitin-proteasome system. a IMS proteins are tagged with a slow maturating mCherry and a fast maturating superfolder green fluorescent protein (sfGFP). The ratio of red (mCherry) to green (sfGFP) intensities correlates with protein stability. b Protein import of Mia40 substrates into the IMS competes with their ubiquitin-dependent degradation in the cytosol
