Fig. 2.

Analysis of Quantified Proteins. (a) Volcano plot showing the 377 proteins (black circles) that changed significantly (p < 0.05) in relative abundance in the injected side as compared to the contralateral non-injected side of wild type mice. The red dots indicate the proteins with >2-fold increase and the blue dots the proteins with >2-fold decrease in relative abundance. (b) Ontological analysis of the 377 proteins, which showed significant changes in relative abundance between the ipsilateral injected side and the contralateral non-injected side in the wild type mice. The circular plot depicts the enriched functional networks of proteins. The outer circle is a scatter plot for each biological term of the logFC of the enriched proteins. Within each network, a protein is depicted as a dot, red for upregulated and blue for downregulated. The size of the inner trapezoids correspond to the adjusted p-value and the colour indicates the z-score as calculated by GOplot algorithm. (c) Representative immunoblots from two independent biological replicates for the significantly changed proteins TH, DAT, and Ddc paired with NSE, PKC-β2 and Akt as unchanged protein controls. The graphs indicate the mean ± sem for protein levels quantified by the SILAM-MS based method and the average of two independent western blot analysis. The relative abundance of NSE, PKC-β2 and Akt did not change in the injected wild type or Snca^−/− mice.