Fig. 3.

Analysis of Quantified Phosphopeptides. (a) Volcano plot showing the 132 phosphorylated proteins (black circles) that changed significantly (p < 0.05) in relative abundance in the injected side as compared to the contralateral non-injected side of wild type mice. The red dots indicate the phosphoproteins with >2-fold increase and the blue dots the phosphoproteins with >2-fold decrease in relative abundance. (b) Motif analysis of the 132 phosphopeptides, which showed a significant change in relative abundance. Enrichment for two motifs was discovered; the XXSP motif, a known target for phosphorylation by MAP kinase and RXXS indicating substrates for CaMK-II kinase. (c) Bubble-plot indicating the cellular components (red) and molecular functions (blue) that were significantly enriched among the 132 phosphopeptides. The area of the displayed circles is proportional to the number of proteins. (d) Construction of a synaptic network from the 377 proteins and 132 phosphopeptides. Black circles indicate proteins that are functionally assigned to synaptic vesicles and showed significant changes in relative abundance. The yellow circles indicate phosphoproteins assigned to synapse and showed significant changes in relative abundance. The gray circles are proteins imported based on known interactions. Physical interactions are depicted as pink edges; associations based on co-localization (blue) and shared protein domains (gold).