Fig. 1.

Expression and function of chimeric Art v 1-specific TCR and HLA-DR1. A, Flow cytometry analysis of TCR Vβ18 and HLA-DR1 expression on CD4⁺ and CD45R/B220⁺ PB lymphocytes isolated from WT, DR1, TCR and TCR-DR1 mice. Markers indicate negative control mAbs, numbers show percentage of cells. n = 4 per group of four analyses. B, Percentages of CD3⁺CD4⁺Foxp3⁺ cells in lymph nodes of WT, DR1, TCR and TCR-DR1 mice. Symbols represent individual mice. C, Dose dependent proliferation of TCR-DR1 splenocytes upon incubation with rArt v 1-protein, or Bet v 1_142–153-peptide (negative control). kcpm, kilo counts per minute of incorporated [³H]-thymidine. Shown are representative SEMs of triplicate cultures of one out of four independent experiments. ***p < .001, Kruskal-Wallis test and Mann-Whitney-U test followed by post hoc Bonferroni correction.