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. 2018 Apr 5;31:66–78. doi: 10.1016/j.ebiom.2018.04.001

Fig. 4.

Fig. 4

IL-2-αIL-2 mAb-dependent in vivo expansion of Treg cells mitigates subsequent sensitization to mugwort extract. A, Scheme for induction and phenotypic analysis of Treg cells induced by IL-2-αIL-2 (1 μg mIL-2 complexed to 5 μg JES6-1 mAb). B, Summary of CD3+CD4+CD25+ PB T cells co-expressing Foxp3 of sham (PBS) or IL-2-αIL-2 treated TCR-DR1 mice obtained on day 6. Symbols represent individual mice. C, Scheme to test the influence of IL-2-αIL-2 complexes on AHR in the presence or absence of αIL-10 or control mAb TCR-DR1 mice. D, IL-2-αIL-2 complex treatment leads to reduced AHR upon aerosol sensitization and challenge with 1% nebulized mugwort pollen extract (3 mg/mouse) of TCR-DR1 mice, which is only modestly reversed by αIL-10 mAb in vivo. Data show the summary (B, D) of 33 IL-2-αIL-2 and 30 PBS treated mice per group (B) and 18 (for IL-2-αIL-2 complex and isotype control mAb, 17 for isotype control mAb, and 9 for IL-2-αIL-2 complex and αIL-10 mAb) treated mice (D) that were analyzed in five (D) and four (except one for αIL-10 mAb treated mice) (D) independent experiments. E, Scheme for the prophylactic treatment of mice with IL-2-αIL-2 complexes and F, corresponding allergen-specific serum Ig levels. G, Scheme for the therapeutic treatment of mice with IL-2-αIL-2 complexes and H, corresponding allergen-specific serum Ig levels. Serum IgE levels of mice treated according to (E) or (F) with IL-2-αIL-2 complexes or PBS used as control substance. Art v 1-specific IgE levels determined by ELISA and expressed in arbitrary units (OD 405 nm) are shown. Symbols represent individual mice. Data show the summary of 10 (G) or 20 (H) (except 18 for PBS treated) mice per group analyzed in two (G) or three (H) independent experiments. *p < .05, **p < .01, ***p < .001, ns, not significant. Student's t-test and ANOVA, followed by Tukey's multiple comparison test.