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. 2018 Jun 15;29(12):1422–1434. doi: 10.1091/mbc.E18-02-0117

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© 2018 Sun and Brodsky “ASCB®,” “The American Society for Cell Biology®,” and “Molecular Biology of the Cell®” are registered trademarks of The American Society for Cell Biology.

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FIGURE 4: — SZ* is sorted into the vacuole through the MVB pathway. (A) Predicted topology of GFP-tagged SZ*. The cytosolic residence of GFP allows for analysis of MVB delivery to the vacuole. (B) GFP cleavage from GFP-SZ* in the wild-type and indicated mutant strains was determined by immonoblotting. G6PD serves as a loading control. (C) The cellular localization of SZ* in both wild-type and vps27Δ cells was determined by fluorescence microscopy. FM4-64 marks the vacuolar membrane. Arrowheads denote the lobes of the vacuole (Bohovych et al., 2016) and the prevacuolar compartment (bottom). Scale bar: 5 μm.