FIGURE 6:

Microtubule-dependent traffic is required for HD maintenance. (A) Confocal plane of the basal membrane of a cell stained for ITGA6 (green) and microtubules (MTs, red) and close-ups of the boxed region (arrowheads highlight colocalization. Scale bar = 10 µm. (B) Normalized signal along the dashed line in the HD channel (ITGA6 staining, green) and MT channel (α-tubulin, red). (C) Cells were incubated with vehicle (CTL) or nocodazole (Noco) before fixation and immunostained for ITGA6. Scale bar = 10 µm. Arrows highlight the differences observed. (D) Quantification of the amount of HDs (from ITGA6 staining) at the basal membrane of cells treated with vehicle or the MT depolymerizing drug nocodazole from three independent experiments; number of cells N = 15, 16, and 11. Student’s t tests: CTL vs. Noco 0.05 µM or Noco 20 µM.