FIGURE 4:

Adhesion of PMNs to respective ligands in a parallel flow chamber test. Adherent resting (A) or fMLF-activated (B) PMNs pretreated with isotype control (black bars) or LFA-1 (red bars)/Mac-1 (blue bars) blocking mAbs on respective ligand-coated substrates after being perfused into the flow chamber and incubated for 5 min without flow. Data are presented as the mean ± SEM of five to nine independent experiments and analyzed with two-way ANOVA (followed by a Holm–Sidak test). Significant differences are indicated by *, p < 0.05; **, p < 0.01; ***, p < 0.001 among various ligands, by ^#, p < 0.05; ^##, p < 0.01; ^###, p < 0.001 between each blocking group and isotype control, or by ^$, p < 0.05; ^$$, p < 0.01; ^$$$, p < 0.001 between LFA-1– and Mac-1–blocking groups.