FIGURE 2:

Phylogenetic tree reconstruction indicates that Tetrahymena condensins are more closely related to condensin I than condensin II. To determine relationships of (A) Cap-D and (B) Cap-H proteins, sequences were aligned with Clustal Omega, and, subsequently, maximum-likelihood trees were generated using PhyML v3.1 with the LG substitution model (Guindon et al., 2010; Sievers et al., 2011). Colored boxes highlight the condensin I (yellow) and condensin II (green) genes. Branch support is indicated by bootstrap values in percentages shown near each branch. The branch-length scale bar represents the estimated number of substitutions per amino acid site. Both Tetrahymena Cap-D homologues group within the Cap-D2 subfamily of HEAT repeat proteins and all four Tetrahymena Cap-H homologues group within the Cap-H kleisin subfamily with high bootstrap support, confirming that all Tetrahymena condensin complexes were derived from condensin I.