FIGURE 6:

Condensin is required for DNA segregation in meiosis. (A) RNAi against SMC2 has no effect on nuclear stretching at meiotic prophase but results in reduced condensation of metaphase chromosomes and extensive DNA bridging at anaphase I and II compared with WT cells. Scale bar equals 5 μm. (B) Enlarged images of metaphase and telophase chromosomes show that chromosomes are less condensed in smc2i cells than in WT cells. Scale bar equals 2 μm. (C) Matings between smc2i and spo11i cells show DNA bridging at anaphase I, indicating that bridge formation is independent of double-strand breaks. Scale bar equals 5 μm. For each sample, 100 meiotic cells were counted and scored as showing either normal meiotic divisions (meiosis I and II categories) or bridged anaphase. Values plotted are the average of two experiments.