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KS-WNK1 puncta do not colocalize with lysosomes. Movie of a HEK-293 cell transfected with C-terminally tagged KSWNK1-mRuby (red), and incubated with LysoTracker Green (green) to stain lysosomes. Live cell images of KS-WNK1 and LysoTracker trafficking were performed using a Zeiss 700 LSM confocal microscope, as described in the Methods. No apparent colocalization, or co-trafficking is observed with KSWNK1 and LysoTracker.
