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COM crystals-induced cell death assay. MDCK cells were treated with various doses of COM crystals for 48 h, and then subjected to Annexin V FITC/PI co-staining followed by flow cytometry analysis. The percentage of cell death = (the number of apoptotic and necrotic cells/the number of total cells) × 100%. Illustrated is a representative of at least three separate experiments and the data were represented as mean ± SD. **p < .01 versus control.
