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. 2018 Jun 19;6:e5037. doi: 10.7717/peerj.5037

Figure 6. Interactions of CmMKKs with CmMPKs in yeast.

Figure 6

The CmMKK ORF fragments were cloned into the pGADT7 (AD) vector in-frame with the GAL4 activation domain, while the CmMPK ORF cDNA fragments were cloned into the pGBKT7 (BD) vector in-frame with the GAL4 binding domain. pGADT7-T-CmMKKx was used as bait, and pGBKT7-CmMPKs were used as prey; (A) pGADT7-CmMKK2; (B) pGADT7-CmMKK3; (C) pGADT7-CmMKK4; (D) pGADT7-CmMKK5; (E) pGADT7-CmMKK6; (F) pGADT7-CmMKK9. In addition, pGADT7-T/pGBKT7-53 was used as a positive control, while pGADT7-T/pGBKT7-Lam was used as a negative control.