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. 2018 Jun 21;6(12):e13733. doi: 10.14814/phy2.13733

Figure 1.

Figure 1

Phosphorylation of eNOS S1177 is enhanced following PI3K activation. Representative western blot analysis of pAKT (S473), pS6 (S235/236), pENOS (S1177), PTEN, and loading control ERK2 for control and PIK3CAH1047R expressing HUVECs (A) and control and PTEN‐KD HUVECs (B) ± hVEGF. HUVECs were treated with hVEGF for 10 min. Total protein levels for AKT, S6, and ENOS are shown in A. Protein densitometry quantification of pENOS (S1177) for control and PIK3CAH1047R expressing HUVECs (C) and control or PTEN‐KD HUVECs (D). Data are presented as mean ± SEM, *=P < 0.05, **=P < 0.001, N = 3. Data are representative of at least two independent infections and three western blot analyses.