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. 2018 Jun 21;6(12):e13733. doi: 10.14814/phy2.13733

Figure 2.

Figure 2

Phosphorylation of eNOS(S1177) is dependent upon mTORc1 activity. (A) Representative western blot analysis of pAKT (S473), pS6 (S235/236), and loading control ERK2 to increasing doses of rapamycin in untransduced HUVECs ± serum for 30 min. Protein densitometry quantification of pAKT (S473) (B) and pS6 (S235/236) (C) and pENOS (S1177) (D) in untransduced HUVECs. For graphical representation of pS6 (S235/236), the +Serum/−Rapamycin condition is shown at 1% of actual fold change to avoid breaking the axis. Representative western blot analysis of pAKT (S473), pS6 (S235/236), pENOS (S1177), and loading control ERK2 in untransduced HUVECs and protein densitometry quantification for pAKT (S473), pS6 (S235/236), and pENOS (S1177) for untransduced (E), PIK3CAH1047R (F), and PTEN‐KD (G) HUVECs ± hVEGF ±0.1 ng/mL rapamycin. Data are presented as percent change when compared to rapamycin untreated conditions (represented by red dotted line. Data are presented as mean ± SEM. N = 3. Data are representative of at least two independent infections and three western blot analyses.