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. 2018 Jun 21;6(12):e13733. doi: 10.14814/phy2.13733

Figure 3.

Figure 3

Activation of mTORc1 by RHEB expression is sufficient to phosphorylate eNOS S1177. (A) Representative western blot analysis of pAKT (S473), total AKT, pS6 (S235/235), total S6, pENOS (S1177), total eNOS and a loading control, ERK2, in control and RHEBS16H‐expressing HUVECs ± hVEGF. (B) Quantification of protein densitometry for pENOS (S1177) normalized to loading control ERK2 for control and RHEBS16H‐expressing HUVECs ± hVEGF. HUVECs were stimulated with hVEGF for 10 min. (C) Quantification of protein densitometry for pAKT (S473), pS6 (S235/236), and pENOS (S1177) for RHEBS16H‐expressing HUVECs +hVEGF +0.1 ng/mL rapamycin. Data are presented as percent change compared to rapamycin untreated conditions. The red dotted line is representative of untreated RHEBS16H protein levels. Graphs are presented as mean ± SEM, *=P < 0.05 N = 3. Data are representative of at least two independent infections and three western blot analyses.