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. 2018 Jan 4;40(1):85–91. doi: 10.1080/0886022X.2017.1419973

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© 2018 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 3. — Effects of mannitol on the apoptosis of HK-2 cells. Flow cytometry assays showed apoptosis of HK-2 cells treated with 0, 100, 250 mM mannitol for 48 h. (A) Shown are representative dot plot of cells stained with Annexin V-FITC/PI following treatment with mannitol for 48 h. (B) Bars represent the cell percentages of early and total apoptotic cells after treatment with different concentrations of mannitol. Note: Compared with the control group, ‘*’ indicated significant difference (p < .05).