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. 2018 Apr 5;40(1):280–288. doi: 10.1080/0886022X.2018.1456462

Figure 1.

Figure 1.

The frequency of peripheral regulatory CD4+ T cells (Tregs) in PBMCs from normal subjects and patients with CKD. Tregs were determined within fresh isolated PBMCs of healthy individuals (n = 35) and chronic kidney disease (CKD) patients not receiving steroids and immunosuppressant therapy (n = 77). (a) A typical example dot plot of the gating strategy CD4+CD25hi for Tregs by flow cytometry-based cell characterizing is given (upper panel). In the lower panel, the frequencies of Tregs within CD4+ T cells are depicted in a graph. (b) A typical example dot plot of the gating strategy CD4+CD25+ FoxP3+ for Tregs by flow cytometry-based cell characterizing is given (upper panel). In the lower panel, the frequencies of Tregs within CD4+ T cells are depicted in a graph. p Values are depicted in the graph. (c) The frequencies of total CD4+ T lymphocytes are depicted in the graph. p Values are depicted in the graph. Study groups were compared using the nonparametric Student’s t-test. *p < .05, **p < .01, NS: no significance, for comparisons between the CKD patients group and the healthy controls using t-tests.