Figure 8.

No tachyphylaxis effect of GPR119 agonist on hypoglycemia-related glucagon response. Normal Wistar rats were predosed without or with compound B (Cmpd B) at a single dose (30 mg/kg) or multiple doses (30 mg/kg each) for 10 days through in-feed drug administration prior to hypoglycemia clamp studies. Blood samples were collected at the indicated time points for measurement of plasma levels of glucose (A), glucose infusion rate (B), glucagon (C), and AUC of glucagon (D). As noted, the increases of glucagon secretion during hypoglycemia mediated by Cmpd B were not significantly different between the groups of rats receiving single vs. multiple doses of Cmpd B. Data are depicted as mean ± SE from 8–10 rats for each group. **P < 0.01; ***P < 0.001 vs. vehicle; NS, P > 0.05.