Figure 6.

Hepatic tissue protein analyses: pAkt (A), GK (B), pGS (C), CLOCK (D), and Per1 (E) proteins expressed relative to total Akt (for pAkt), cyclophilin B (for GK, CLOCK, and Per1), or total GS (for pGS). Individual data are depicted by unfilled circles, with dark gray lines indicating the median for each treatment. Control (CON) samples (n = 3) were taken from the SAL_no-clamp dogs described in our previous publication (6); see research design and methods (n = 5 for GLC and n = 6 for INS). The amGLC and amINS data were derived from the two dogs in each treatment group that underwent the AM clamp and the subsequent nonclamp period, with tissue collection occurring at 360 min. Statistical analyses were done only where treatments included at least n = 3. Groups marked with different letters differed significantly (P < 0.05).