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. 2018 Apr 16;67(7):1332–1344. doi: 10.2337/db17-1352

Figure 2.

Figure 2

Generation of βDoc2b-dTg mice. A: Schematic design of RIP-rtTA−/+ mice crossed to new TRE-Doc2b−/+ mice. B: rDoc2b-myc mRNA, in islets isolated from female double transgenic (βDoc2b-dTg) mice. Dox-induced expression of Doc2b-myc and endogenous Doc2b protein in isolated islets (C) and lack of overexpression of rDoc2b-myc mRNA in the hypothalamus or cerebellum of the βDoc2b-dTg mice (D). IB, immunoblot. E: Immunofluorescence imaging of Doc2b overexpression in the islets of βDoc2b-dTg mice treated with or without Dox. Arrows denote the same position in each horizontal panel to show positive or negative c-Myc staining within a β-cell. Shown are the mean ± SD of at least three mice per group. *P < 0.05. Bar = 50 µm.