Fig. 2.

Salivary miRNA expression of HFMD in the salivary miRnome screen. (A) PCA plot based on miRNA expression values across pools (n = 3 each). (B) Heat map showing differentially expressed miRNA gene expression pattern across different samples (n = 3). Volcano plot was used to illustrate distribution of significant and non-significant miRNA in Singapore HFMD Cohort 1 screen in C. Healthy Vs EV71 (n = 6) and D. Healthy Vs CA6 (n = 6). Red colour represent population with absolute 4-fold change while significant miRNAs were represented in yellow or green while red is non-significant. Student t-test (non-parametric) was performed and HITs were determined using absolute fold change of >4-fold and p-value < 0.05.