Table 4.
Surface marker expression by adHER2/neu DCs manufactured from healthy donor monocytes using AB and patient #13 plasma.
| Monocyte Donor |
CD340 (%) |
CD197 (%) |
CD14 (%) |
CD86 (%) |
CD83 (%) |
CD38 (%) |
CD54 (%) |
CD340 (MFI) |
HLA DR (MFI) |
|
|---|---|---|---|---|---|---|---|---|---|---|
| 1 | 90.8 | 11.9 | 21.0 | 99.0 | 82.2 | 96.3 | 99.2 | 7571 | 3035 | |
| AB | 2 | 85.6 | 11.0 | 41.2 | 99.1 | 94.4 | 99.0 | 99.5 | 5238 | 4732 |
| 3 | 94.2 | 2.6 | 27.2 | 98.9 | 73.6 | 94.3 | 98.7 | 6261 | 10402 | |
| Aver ± SD | 90.2±4.3 | 8.5±5.1 | 29.8±10.3 | 99.0±0.1 | 83.4±10.5 | 96.5±2.4 | 99.1±0.4 | 6356±1169 | 6056±3857 | |
| 1 | 16.4 | 26.0 | 23.5 | 99.0 | 93.0 | 99.4 | 98.9 | 686 | 7074 | |
| Pt #13 | 2 | 13.2 | 38.7 | 34.6 | 99.4 | 98.7 | 99.9 | 99.5 | 404 | 5534 |
| 3 | 23.4 | 31.3 | 26.8 | 97 | 93.4 | 99.1 | 96.1 | 978 | 6811 | |
| Aver ± SD | 17.7±5.2 | 32.0±6.4 | 28.3±5.7 | 98.5±1.3 | 95.0±3.2 | 99.5±0.4 | 98.2±1.8 | 689±287 | 6473±823 | |
| 1 | 31.8 | 19.1 | 28.3 | 99.3 | 93.5 | 99.8 | 99.5 | 1105 | 9092 | |
| Mix | 2 | 29.9 | 30.4 | 39.6 | 99.7 | 96.7 | 99.9 | 99.8 | 856 | 8334 |
| 3 | 46.6 | 10.9 | 34.8 | 98 | 88.6 | 99.4 | 96.8 | 1923 | 5794 | |
| Ave±SD | 36.1±9.1 | 20.1±9.8 | 34.2±5.7 | 99.0±0.9 | 92.9±4.1 | 99.7±0.3 | 98.7±1.7 | 1294±558 | 7740±1727 | |
| AB vs Auto | 2.06×10−04 | 3.79×10−02 | N.S. | N.S. | N.S. | N.S. | N.S. | 1.18×10−02 | N.S. | |
| AB vs Mix | 3.90×10−03 | 9.63×10−02 | N.S. | N.S. | N.S. | N.S. | N.S. | 1.87×10−02 | N.S. | |
| Auto vs Mix | 1.67×10−02 | 1.09×10−01 | 2.91×10−02 | N.S. | N.S. | N.S. | 4.72×10−02 | N.S. | N.S. |
AB = AB plasma
Auto = autologous patient #13 plasma
Mix = equal mixture of AB and patient #13 plasma
Surface marker expression was measured by flow cytometry