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. Author manuscript; available in PMC: 2018 Jun 22.
Published in final edited form as: Cell. 2011 Mar 4;144(5):703–718. doi: 10.1016/j.cell.2011.02.003

Figure 3. PTPN12 Suppresses Transformation by Inhibiting HER2/EGFR Signaling.

Figure 3

(A) EGFR and HER2 depletion in TLM-HMECs. TLM-HMECs expressing control, EGFR, or HER2-targeting shRNAs were analyzed by western blotting for EGFR, HER2, and vinculin (loading control) as indicated.

(B) EGFR and HER2 RTKs are required for cellular transformation upon PTPN12 depletion. TLM-HMECs encoding an inducible PTPN12-shRNA were transduced with the indicated shRNAs and assessed for anchorage-independent proliferation ± dox.

(C) SHC depletion in HMECs. TLM-HMECs expressing control or SHC-targeting shRNAs were analyzed by western for SHC and vinculin as indicated.

(D) SHC is required for cellular transformation upon PTPN12 depletion. TLM-HMECs encoding an inducible PTPN12-shRNA were transduced with the indicated shRNAs and assessed for anchorage-independent proliferation ± dox.

(E) HER2/EGFR inhibitors block PTPN12 depletion-induced transformation. TLM-HMECs expressing the indicated shRNAs were assessed for anchorage-independent growth ± a HER2/EGFR inhibitor (lapatinib).

(F) Transformation by PTPN12 inactivation requires MAPK signaling. TLM-HMECs expressing the indicated shRNAs were assessed for anchorage-independent proliferation ± a MEK inhibitor (U0126).

Error bars represent standard error.