Skip to main content
. Author manuscript; available in PMC: 2018 Jun 22.
Published in final edited form as: Cell. 2011 Mar 4;144(5):703–718. doi: 10.1016/j.cell.2011.02.003

Figure 6. PTPN12 Suppresses Growth and Metastasis of TNBC Cells.

Figure 6

(A) PTPN12 expression is reduced in TNBC cell lines. PTPN12 protein levels were quantified by western in HMECs, TNBC cells, and HER2-amplified breast cancer cells as indicated.

(B) Reconstituting PTPN12 expression suppresses proliferation in PTPN12-deficient breast cancer cells. TNBC cells expressing low endogenous PTPN12 were transduced with equivalent multiplicity of infection (moi) of retrovirus encoding eGFP (control) or PTPN12 cDNAs and analyzed for macroscopic colony formation in vitro.

(C) Reconstituting PTPN12 expression suppresses proliferation in PTPN12-deficient breast cancer cells. Quantification of colony number from cells in (B).

(D) PTPN12 expression is reduced in aggressive lung metastatic subpopulation of TNBC MDA-MB231 cells. PTPN12 protein expression was assessed in MDA-MB231 breast cancer cells (231-parent) and in MDA-MB231-LM2 subpopulation that exhibits enhanced primary and lung metastatic tumor growth. MDA-MB231-LM2 cells were engineered with an inducible PTPN12-cDNA (LM2-IP cells) that expresses similar PTPN12 levels as parental MDA-MB231 cells upon addition of dox.

(E) Restoring PTPN12 expression suppresses primary tumor growth in aggressive TNBC cells. Cells from (D) were transplanted in the mouse mammary gland and monitored for primary tumor growth in the presence or absence of dox (n = 12 for each group).

(F) Restoring PTPN12 expression suppresses lung metastatic growth in aggressive TNBC cells. Cells from (D) were tail vein injected and monitored for lung metastatic growth (via luminescence detection) in the presence or absence of dox (n = 6 for each group). Representative +dox and −dox images and quantification are shown in upper and lower panels, respectively.

(G) Restoring PTPN12 expression suppresses lung metastatic growth in aggressive TNBC cells. Cells from (D) were tail vein injected (as in F) in the presence or absence of dox (n = 7) and analyzed for lung metastatic lesions via standard H&E.

Error bars represent standard error.