Fig. 3.

OCT images of OV after SE removal and continued culture. Dashed red lines indicate the boundary between the SE and OV; dashed yellow lines indicate OV shape after overnight culture. (A, A’, A”) SE removal before placode formation had little effect on OV shape immediately after dissection (A, A’, Phase 0, HH12+) but inhibited subsequent invagination (A”). (B, B’, B”) Removing the SE after placode formation (B, B’, Phase 2, HH14−) caused immediate loss of concavity in most embryos (n = 24/34), but invagination still occurred in some embryos that were cultured overnight (n = 4/6) (B”), as reported by Hyer et al. (2003). Scale bar 100 µm