Figure 2.

A) Post gene transfer tissue sections from EDB muscles for subjects 1, 2, and 3. The left panel shows α-SG gene expression on the side of vector injection (T) compared to muscle from opposite side (C)(scale bar = 100 μm). B) Western blots from all three cases show increased α-SG gene expression on the side of gene transfer compared to the contralateral side (treated on left, control on right) showing residual gene expression from mutant protein. α-SG is normalized to actin (lower band). C) β-Sarcoglycan staining demonstrates restoration on the side of gene transfer. Other sarcoglycans (γ and δ) were also restored (not shown). Muscle from subject (#2) is shown (scale bar = 100μm). D) Densitometry measurements show α-SG gene expression increased 4 to 5 fold on the side of vector injection.