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. Author manuscript; available in PMC: 2019 Jun 7.
Published in final edited form as: Vaccine. 2018 May 5;36(24):3513–3521. doi: 10.1016/j.vaccine.2018.04.067

Figure 1.

Figure 1

Construction and purification of S. aureus CTA2/B chimeric mucosal vaccines. (A) ribbon diagram of Vibrio cholerae cholera toxin [28], (B) expression of the IsdA-CTA2/B chimera from E.coli + pLR001, (C) SDS-PAGE of d-galactose affinity-purified IsdA-CTA2/B (IsdA-CTA2 = 38kD, CTB = 11kD) vaccine preparation, (D) expression of the ClfA-CTA2/B chimera from E.coli + pLR003, and (E) SDS-PAGE of d-galactose affinity-purified ClfA-CTA2/B (ClfA-CTA2 = 37kD, CTB =11kD) vaccine preparation.