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. Author manuscript; available in PMC: 2019 Aug 15.
Published in final edited form as: Dev Biol. 2018 May 21;440(2):152–166. doi: 10.1016/j.ydbio.2018.05.015

Figure 3. Mutant EGL is largely agranular due to severely reduced CGNP proliferation.

Figure 3

(A) Immunohistochemistry for pH3 (brown) at P3 and P5 in WT and Smocko mutants. (B) Quantification of pH3 staining as a percentage of total EGL cells. At P3, a 19.5% decrease (n=3, p=0.0314) in pH3 was observed. At P5, a 51.1% decrease in pH3 (n=3, p=0.0166) was observed. (C) Immunohistochemistry for p27Kip1 (brown) at P3 and P5 in WT and Smocko mutants. (D) Quantification of p27Kip1 staining as a percentage of total EGL cells. At P3, an 18.9% increase (n=3, p=0.0052) in p27Kip1-positive cells was observed. At P5, a 39.2% increase (n=3, p=0.0026) in p27Kip1-positive cells was observed. Sections in A and C were counter stained with hematoxylin to highlight the nuclei (blue). (E) Immunofluorescence staining of Ki67 (green) and Tag1 (red) at P5 in WT and Smocko mutants. (F) Quantification of Tag1 staining as a percentage of total EGL cells. A 56% increase (n=3, p=0.0615) in Tag1-positive cells was observed. In contrast, a 28.3% decrease (n=3, p=0.0128) in Ki67-positive cells was observed. Abbreviations: EGL, external granular layer. Scale bar: 20 µm.