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. Author manuscript; available in PMC: 2019 Aug 15.
Published in final edited form as: Dev Biol. 2018 May 21;440(2):152–166. doi: 10.1016/j.ydbio.2018.05.015

Figure 8. Smocko mutants exhibit aberrant Wnt signaling.

Figure 8

(A–B’) β-galactosidase immunohistochemistry in P4 TNCYFP-CreER; SmoF/−; BAT-Gal mice (B, B’) shows the presence of ectopic β-gal+ cells in the EGL of mutant mice (arrows), indicating that Smocko mutant mice have enhanced Wnt signaling in CGNPs. (C–D’) In situ hybridization for Sfrp1 in Smocko mutants (D, D’) at P4 indicates a downregulation of the mRNA in the EGL. (E) Western blotting of lysates of freshly isolated CGNPs indicates a downregulation of Sfrp1 expression. Abbreviations: EGL, external granular layer. CGNPs, cerebellar granular neuron precursors. Scale bar, 200µm and 20 µm.