Fig 5. NK-4 suppresses the mRNA expression of the Th2-associated transcription factors GATA-3 and NFATc1.
Total RNA was extracted from D10.G4.1 cells (2 x 106 cells) stimulated with immobilized anti-CD3ε mAb (8 μg/ml) in the presence or absence (control) of NK-4 for 6 h at 37°C. First-strand cDNA was synthesized using reverse transcriptase, as described in the Materials and Methods. Expression levels of GATA-3 (A), NFATc1 (B) and c-Maf (C) were analyzed by real-time PCR. 18S rRNA expression levels were used for normalization. Results are given as changes in gene expression relative to the mean values of the control. Results are the means ± S.D. of three independent experiments. *p < 0.05, compared with control cultures.